Paper 3 · Paper 5 · Investigations

Run the experiment. Explain the result.

Water potential and enzyme activity are core to 9700 practical and planning papers. Weigh potato cylinders, find the concentration with no change in mass, time amylase at different temperatures, then practise the control variables and explanations examiners expect.

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What examiners look for

Precision

Readings recorded to the precision of the instrument and calculated values to sensible significant figures.

Water potential

The concentration giving no change in mass has the same water potential as the cells; read it from the line where it crosses 0%.

Rates

Rate = 1 ÷ time, in s⁻¹, with the optimum taken from the data.

Control variables

Temperature with a thermostatically controlled water bath, pH with a buffer, and fixed volumes, concentrations and sizes.

Explanations

Osmosis described in terms of water potential; denaturation in terms of the active site.

Habits that save marks

The marking follows the conventions in published Cambridge mark schemes. The experiments, readings and wording on this page are our own.