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Transport in plants

A-Level Biology 9700 · Topic 7 revision notes

Everything from the 4 lessons of this topic on a few pages: the key idea of each lesson, the diagram to remember, the points to learn and the traps examiners set. Read it, cover it, test yourself.

  1. Xylem and phloem: structure and distribution
  2. From soil to xylem, and up the stem
  3. Transpiration and xerophytic leaves
  4. Translocation: loading the phloem and mass flow

1Xylem and phloem: structure and distribution

Syllabus 7.1

In a herbaceous dicotyledonous plant the xylem carries water and mineral ions up from the roots, and the phloem carries assimilates such as sucrose and amino acids from sources to sinks. Their positions differ: in the root the xylem is in the centre, often X or star shaped, with phloem between its arms; in the stem the vascular bundles form a ring near the outside, with xylem on the inner side and phloem on the outer side of each bundle; in the leaf midrib and veins the xylem is on the upper side and the phloem on the lower side. Xylem vessel elements are dead, empty and joined end to end, with lignified walls and pits. Sieve tube elements are living but have no nucleus and very little cytoplasm, with sieve plates between them; each is kept alive by a companion cell with a nucleus, many mitochondria and many plasmodesmata.

rootstemleaf (midrib) xylem in the centre,phloem between its arms bundles in a ring: xylem inside,phloem outside xylem on the upper side,phloem on the lower side xylem phloem endodermis ring
Fig. 1: plan diagrams of transverse sections of a dicotyledonous root, stem and leaf, showing the distribution of xylem and phloem

Position, then structure.

  • Root: xylem central, X or star shape; phloem between the arms.
  • Stem: ring of bundles; xylem inside, phloem outside, cambium between.
  • Leaf: xylem upper, phloem lower in each vein.
  • Xylem dead, empty, lignified; sieve tube living, no nucleus, sieve plates.

Watch out!

A plan diagram shows the outlines of tissues only: no individual cells, no shading, clear continuous lines, proportions that match the specimen and label lines drawn with a ruler. Drawing cells in a plan diagram loses marks.

Xylem vessel elements are dead, so they cannot actively transport anything; water moves up them passively. Sieve tube elements are alive even though they lack a nucleus. Mixing these up is a common error.

2From soil to xylem, and up the stem

Syllabus 7.2

Mineral ions and some organic compounds are carried around a plant dissolved in water. Water enters root hair cells by osmosis and crosses the cortex by two routes: the apoplast pathway through the permeable cellulose cell walls and spaces between cells, and the symplast pathway through the cytoplasm, passing from cell to cell through plasmodesmata. At the endodermis a band of waterproof suberin, the Casparian strip, blocks the apoplast, so all water must pass through the endodermal cells, letting the plant control which ions reach the xylem. In the xylem, water molecules form hydrogen bonds with each other (cohesion) and with the cellulose of the walls (adhesion). As water is lost from the leaves, the continuous column is pulled up under tension: the transpiration pull. Lignin keeps the vessels from collapsing and makes their walls waterproof.

root haircell cortexcortex endodermisxylem Casparian strip lignified walls;water enters at pits plasmodesmata apoplast: through cellulose cell walls symplast: through cytoplasm and plasmodesmata
Fig. 1: the apoplast and symplast pathways across the root cortex, with the Casparian strip blocking the apoplast at the endodermis

Two routes, one column.

  • Apoplast: cellulose walls and spaces, no membranes, fast.
  • Symplast: cytoplasm and plasmodesmata.
  • Casparian strip (suberin) blocks the apoplast at the endodermis: control of ions.
  • Cohesion water to water, adhesion water to cellulose, both hydrogen bonds; tension from transpiration.

Watch out!

The Casparian strip blocks the apoplast only. It does not stop water reaching the xylem: water simply has to go through the cytoplasm of the endodermal cells (the symplast) for that step. Never write that the Casparian strip "stops water entering the xylem".

Water moves up the xylem passively: the energy comes from the Sun evaporating water in the leaf, not from ATP in the xylem. Xylem vessel elements are dead and cannot pump anything.

3Transpiration and xerophytic leaves

Syllabus 7.2

Transpiration is the loss of water vapour from the aerial parts of a plant, mainly the leaves. Water evaporates from the wet surfaces of mesophyll cell walls into the air spaces inside the leaf, and the water vapour then diffuses out through open stomata, down a water potential gradient, to the drier air outside. Low humidity, high temperature, wind and the light that opens stomata all increase the rate. The water lost is replaced from the xylem, which is what pulls the water column up the plant. Xerophytes are plants adapted to dry places. Marram grass reduces transpiration with rolled leaves (rolled by hinge cells), sunken stomata in grooves on the inner surface, hairs that trap moist air and a thick waxy cuticle on the exposed outer surface. Each adaptation either keeps humid air next to the stomata, so the water potential gradient is shallow, or makes the surface less permeable.

humid air trapped inside thick waxy cuticle, outer surface only: less evaporation stomata sunken in grooves on the inner surface hairs trap still, moist air and slow diffusion leaf rolled: humid air kept next to the stomata hinge cells lose water and shrink, so the leaf rolls up marram grass leaf, transverse section
Fig. 1: an annotated drawing of a transverse section of a rolled marram grass leaf, a xerophyte

Two steps, then the gradient.

  • Evaporation from mesophyll cell walls into air spaces.
  • Diffusion of water vapour out through stomata, down a water potential gradient.
  • Humidity down, temperature, wind and light up: rate up.
  • Xerophytes: trap humid air near stomata or thicken the cuticle.

Watch out!

Say that water evaporates from the internal surfaces (the mesophyll cell walls) and then water vapour diffuses out through the stomata. Writing that "water evaporates out of the stomata" or that "water diffuses out of the leaf" merges two steps and loses marks.

Xerophyte adaptations reduce water loss; they do not stop it. The stomata still have to open for carbon dioxide, so some transpiration always happens.

4Translocation: loading the phloem and mass flow

Syllabus 7.2

Assimilates, mainly sucrose and amino acids, are carried dissolved in water in phloem sieve tubes from sources, where they are made or released (photosynthesising leaves, storage organs being emptied), to sinks, where they are used or stored (roots, growing tips, fruits, storage organs being filled). At the source, companion cells use ATP to drive proton pumps that move hydrogen ions out of the cell. The ions diffuse back in through cotransporter proteins, carrying sucrose with them against its concentration gradient, and the sucrose passes into the sieve tube through plasmodesmata. This lowers the water potential in the sieve tube, so water enters from the xylem by osmosis and the hydrostatic pressure rises. At the sink, sucrose is removed, water leaves and the pressure is lower, so the sap moves by mass flow down the pressure gradient from source to sink.

SOURCEphotosynthesising leaf SINKroot, fruit, growing tip sievetube xylem mass flow of sap down the pressure gradient sucrose loaded sucrose unloaded water in by osmosis high pressure water out by osmosis lower pressure loading: companion cells use ATP to pump H⁺ out; H⁺ diffuses back in through cotransporter proteins, carrying sucrose with it
Fig. 1: mass flow in a phloem sieve tube from source to sink, driven by a hydrostatic pressure gradient

Energy at the source, flow down the gradient.

  • Source makes or releases sucrose; sink uses or stores it.
  • Proton pump uses ATP; cotransporter uses the H+ gradient.
  • Sucrose in, water potential down, water in, pressure up.
  • Mass flow from high to low hydrostatic pressure, source to sink.

Watch out!

The cotransporter does not use ATP directly. ATP is used by the proton pump; the cotransporter uses the energy of H+ ions diffusing back down their gradient. Saying "sucrose is actively pumped by the cotransporter using ATP" is a common error.

Mass flow is a passive movement down a pressure gradient. The energy is spent at the source, on loading. Do not write that sieve tubes "pump" sap or that sucrose diffuses all the way to the sink.

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