Cambridge IGCSE · Topic 11.1
IGCSE Biology 0610 / Topic 11 / IGCSE

In with oxygen, out with carbon dioxide.

Breathing changes the air: the air we breathe out has less oxygen, more carbon dioxide and more water vapour than the air we breathe in. This lesson tests the difference with limewater, measures how exercise changes the rate and depth of breathing and (Extended) explains how the ribs, intercostal muscles and diaphragm move air, and how the brain responds to carbon dioxide.

The key idea

Expired air contains less oxygen (about 16% instead of 21%), more carbon dioxide (about 4% instead of 0.04%) and more water vapour than inspired air, and turns limewater milky much faster. During physical activity breathing becomes faster and deeper. Extended: muscles change the volume of the thorax, which changes the pressure, so air moves in and out; extra carbon dioxide in the blood during exercise is detected by the brain, which increases the rate and depth of breathing.

breathing in ribs up and out, diaphragm flattens volume up, pressure down: air in breathing out ribs down and in, diaphragm domes volume down, pressure up: air out
Fig. 1: breathing in enlarges the thorax and lowers the pressure inside the lungs, so air flows in; breathing out reverses the changes
Section 01

Volume up, pressure down.

Extended. The lungs cannot move themselves; they follow the walls of the thorax. Press Breathe in, Breathe out and Forced breath out, and watch the external intercostal muscles (red), the internal intercostal muscles (blue) and the diaphragm.

Breathing in (inspiration)Breathing out (expiration)
external intercostal musclescontractrelax
internal intercostal musclesrelaxcontract in forced breathing out
ribsmove up and outmove down and in
diaphragmcontracts and flattensrelaxes and domes upwards
volume of thoraxincreasesdecreases
pressure in lungsfalls below atmosphericrises above atmospheric
airflows inflows out
Examiner trap

Air does not rush in and then make the chest expand. The order is: muscles contract, volume increases, pressure falls, then air flows in. And the diaphragm flattens when it contracts; it domes upwards when it relaxes.

Section 02

Inspired and expired air.

In the limewater apparatus, air breathed in is drawn through tube A and air breathed out bubbles through tube B. Press Breathe in and out 10 times and compare the two tubes, then read the composition chart.

GasInspired airExpired airExtended: why
oxygen21%16%cells use oxygen in aerobic respiration, so some is absorbed into the blood
carbon dioxide0.04%4%respiration produces carbon dioxide, which diffuses from the blood into the alveoli
water vapourvariesmore (saturated)water evaporates from the moist lining of the alveoli
Examiner trap

Expired air still contains plenty of oxygen (about 16%), which is why rescue breaths work. Do not write that expired air has "no oxygen" or is "all carbon dioxide".

Section 03

Exercise: faster and deeper.

Choose resting, walking, jogging or sprinting and watch the spirometer trace. The breathing rate is the number of breaths per minute; the depth is the volume of each breath.

ActivityRate / breaths per minDepth / dm3
resting120.5
walking180.9
jogging281.8
sprinting402.6
Examiner trap

In the Extended explanation the trigger is the rise in carbon dioxide in the blood, detected by the brain. Do not say the lungs detect a lack of oxygen, or that the muscles decide how fast to breathe.

Stage 1 · Learn

Check what the sim just showed you

Four quick checks. Each correct answer earns XP and records this skill on your profile.

Quick check+10 XP

In the ventilation tab, what happens to the pressure in the lungs as you press Breathe in?

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Which muscles light up (blue) when you press Forced breath out?

Quick check+10 XP

After 10 breaths in the limewater apparatus, which tube has turned milky?

Quick check+10 XP

In the exercise tab, what are the breathing rate and depth when sprinting?

Stage 2 · Exam

Exam-style questions

Unlocks once the four checks above are done. Worth more XP, written to IGCSE Paper 2 and Paper 4 standard.

Finish the four checks above to unlock the exam questions
Exam style+20 XP

Which row correctly compares expired air with inspired air?

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Extended: during breathing in, which changes happen?

Exam style+20 XP

Extended: why does expired air contain more water vapour than inspired air?

Exam style+20 XP

Extended: during exercise the rate and depth of breathing increase because:

Skill unlocked

Breathing and exercise, mastered.

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Go deeper · practice
Six original Cambridge-style questions
Limewater tests on inspired and expired air, air composition, breathing rate and depth during exercise, and (Extended) ventilation mechanics and carbon dioxide control.